Table 1 Thermodynamic data for binding of H3K4me3 to KDM5A PHD3.

From: Mechanism of biomolecular recognition of trimethyllysine by the fluorinated aromatic cage of KDM5A PHD3 finger

 

Kd (nM)

ΔG° (kcal mol−1)

ΔH° (kcal mol−1)

−TΔS° (kcal mol−1)

BL21 WT-KDM5A

54 ± 6

−9.9 ± 0.1

−11.6 ± 0.1

1.7 ± 0.2

AUX WT-KDM5Aa

48 ± 8

−10.0 ± 0.1

−11.9 ± 0.1

1.9 ± 0.1

5F-KDM5A

49 ± 12

−10.0 ± 0.1

−10.9 ± 0.1

0.9 ± 0.3

6F-KDM5A

57 ± 17

−9.9 ± 0.2

−12.1 ± 0.1

2.2 ± 0.3

5,6diF-KDM5A

70 ± 15

−9.8 ± 0.1

−11.7 ± 0.2

1.9 ± 0.3

  1. 10-mer H3K4me3 peptide (ARTKme3QTARS) was used. Values obtained from 7–9 repeated ITC experiments. BL21 and AUX indicate the expression system used for WT protein expression with BL21 corresponding to the Rosetta BL21 (DE3) pLysS and AUX to the Castellani and Chalmers auxotrophic strains, respectively. The stoichiometry (H3K4me3:KDM5A, n) = 0.97–1.03.
  2. aMeasured in replicate.
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