Fig. 3: Oxidative folding analysis of the synthetic proteins.
From: Diselenide crosslinks for enhanced and simplified oxidative protein folding
The anaerobic oxidative folding at pH 8.7 in the presence of 150 µM GSSG and 30 µM of a WT-Hir, b Hir(C16U/C28U), c Hir(C22U/C39U), d Hir(C6U/C14U) and e Hir(C6U/C16U). # denotes an impurity with unknown mass. 1-SS, 2-SS and 3-SS represent the number of disulfide (or diselenide) crosslinks in the intermediates. f Retention times of isolated native states of WT-Hir and the seleno-variants.
