Fig. 4: Comparison between the synthetic proteins; aerobic folding and inhibition of thrombin.
From: Diselenide crosslinks for enhanced and simplified oxidative protein folding
a Kinetic traces of the aerobic folding in the absence of GSSG and with 30 µM WT-Hir (blue circles) or Hir(C16U/C28U) (red diamonds) at pH 8.7. The lines connecting the data points are shown only for illustrative purposes and do not represent a data fit. The data points are based on two repetitive experiments and the error was within 5%. b Inhibition activity of WT-Hir and Se-Hir analogues. The inhibition of bovine thrombin by WT-Hir (blue circles), Hir(C16U/C28U) (red diamonds), Hir(C22U/C39U) (brown circles), Hir(C6U/C14U) (blue filled squares) and Hir(C6U/C16U) (open squares). The data were fitted as described in the Supplementary Methods 4.6 to give the apparent KI values indicated.
