Fig. 1: Influence of LZNV on the thermostability and function of YePEPT.

a Thermal shift assay using YePEPT^WT and YePEPT^K314A, and LZNV. The T_i-values of YePEPT^WT and YePEPT^K314A were determined at different concentrations of LZNV (200 µM, 400 µM and 800 µM). ΔT_i-values at specified LZNV concentrations were calculated by subtraction of the T_i-value in the absence of LZNV. Data points represent mean ± SD of at least three independent experiments. b Kinetics of [³H]Ala-Ala uptake in E. coli cells transformed with the YePEPT^K314A construct. Data points represent uptake of [³H]Ala-Ala normalised to V_max ± SEM of three independent experiments, each at least in triplicate. c IC₅₀ determination of YePEPT^K314A for LZNV by heterologous competition. Data points represent uptake of [³H]Ala-Ala normalised to the uninhibited signal ± SEM of three independent experiments, each at least in triplicates. Numbers in brackets below K_m- and IC₅₀-values represent 95% confidence intervals. If not visible, error bars are smaller than symbols.