Fig. 6: Impact of BSO-OxOAc as single agent and in combination with the reducing agents NAC (100 µM) and AA (50 µM) on the clonogenic potential of HCT116 cells and the subline with acquired oxaliplatin resistance (HCT116/OxR).
From: A platinum(IV) prodrug strategy to overcome glutathione-based oxaliplatin resistance
Sparsely seeded cells (1 × 103/24-well plate well) were exposed for 10 days to the indicated compounds and derived cell clones stained with crystal violet, photographed, and results evaluated by ImageJ software as described in the Methods section. One respective experiment out of three performed in duplicate is shown under (a) and the respective evaluation under (b), depicted as mean ± SD. Statistical significance was tested using two-way ANOVA. In all cases: *p < 0.05; **p < 0.01; ***p < 0.001.
