Fig. 5: Perfluoroaryl-stapled and alanine-substituted peptides demonstrate high-affinity binding.

a Perfluoroaryl-stapled and alanine-substituted peptides 35–40 with low nanomolar binding affinity were generated at tolerable (i, i + 4) stapling sites (Fig. 3c). b Structure of peptide 39, alanine is colored in purple and stapling sites are colored in blue. c Example competition binding curves of peptides. C* = cysteine stapled with hexafluorobenzene.