Fig. 2: Screening for IREDs using the increasing-molecule-volume-screening approach.
From: Actinomycetes-derived imine reductases with a preference towards bulky amine substrates
The number of enzyme hits is shown above the structure of the corresponding secondary amine. The black color scale in each tile is representative of the number of hits generated through the screening with the given substrate. The standard assay mixture (50 μL) contained 5.0 mM ketone, 5.0 mM amine, 1.0 mM NADP+, GDH (0.2 mg ml−1), D-glucose (30 mM), 100 mM potassium phosphate buffer (pH 7.0), and 1 mg mL−1 IREDs. The conversion of reductive amination products was determined by comparison with UV-Vis absorptions of synthesized standard reference materials. The enzymes (IR-G02, 21, and 35) with red triangle block are potent IREDs.
