Table 1 ThT buffers prepared with the FORMULATOR® - screen builder in a 96-deep well plate.
From: Multivariate effects of pH, salt, and Zn2+ ions on Aβ40 fibrillation
ThT buffers | 20 mM potassium phosphate with 40 μM ThT in the absence of 40 μM Zn | 20 mM potassium phosphate with 40 μM ThT in the presence of 40 μM Zn | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | pH 6.5 0 M NaCl | pH 6.8 0 M NaCl | pH 7.1 0 M NaCl | pH 7.4 0 M NaCl | pH 7.7 0 M NaCl | pH 8.0 0 M NaCl | pH 6.5 0 M NaCl | pH 6.8 0 M NaCl | pH 7.1 0 M NaCl | pH 7.4 0 M NaCl | pH 7.7 0 M NaCl | pH 8.0 0 M NaCl |
B | pH 6.5 0.0143 M NaCl | pH 6.8 0.0143 M NaCl | pH 7.1 0.0143 M NaCl | pH 7.4 0.0143 M NaCl | pH 7.7 0.0143 M NaCl | pH 8.0 0.0143 M NaCl | pH 6.5 0.0143 M NaCl | pH 6.8 0.0143 M NaCl | pH 7.1 0.0143 M NaCl | pH 7.4 0.0143 M NaCl | pH 7.7 0.0143 M NaCl | pH 8.0 0.0143 M NaCl |
C | pH 6.5 0.0286 M NaCl | pH 6.8 0.0286 M NaCl | pH 7.1 0.0286 M NaCl | pH 7.4 0.0286 M NaCl | pH 7.7 0.0286 M NaCl | pH 8.0 0.0286 M NaCl | pH 6.5 0.0286 M NaCl | pH 6.8 0.0286 M NaCl | pH 7.1 0.0286 M NaCl | pH 7.4 0.0286 M NaCl | pH 7.7 0.0286 M NaCl | pH 8.0 0.0286 M NaCl |
D | pH 6.5 0.0429 M NaCl | pH 6.8 0.0429 M NaCl | pH 7.1 0.0429 M NaCl | pH 7.4 0.0429 M NaCl | pH 7.7 0.0429 M NaCl | pH 8.0 0.0429 M NaCl | pH 6.5 0.0429 M NaCl | pH 6.8 0.0429 M NaCl | pH 7.1 0.0429 M NaCl | pH 7.4 0.0429 M NaCl | pH 7.7 0.0429 M NaCl | pH 8.0 0.0429 M NaCl |
E | pH 6.5 0.0571 M NaCl | pH 6.8 0.0571 M NaCl | pH 7.1 0.0571 M NaCl | pH 7.4 0.0571 M NaCl | pH 7.7 0.0571 M NaCl | pH 8.0 0.0571 M NaCl | pH 6.5 0.0571 M NaCl | pH 6.8 0.0571 M NaCl | pH 7.1 0.0571 M NaCl | pH 7.4 0.0571 M NaCl | pH 7.7 0.0571 M NaCl | pH 8.0 0.0571 M NaCl |
F | pH 6.5 0.0714 M NaCl | pH 6.8 0.0714 M NaCl | pH 7.1 0.0714 M NaCl | pH 7.4 0.0714 M NaCl | pH 7.7 0.0714 M NaCl | pH 8.0 0.0714 M NaCl | pH 6.5 0.0714 M NaCl | pH 6.8 0.0714 M NaCl | pH 7.1 0.0714 M NaCl | pH 7.4 0.0714 M NaCl | pH 7.7 0.0714 M NaCl | pH 8.0 0.0714 M NaCl |
G | pH 6.5 0.0857 M NaCl | pH 6.8 0.0857 M NaCl | pH 7.1 0.0857 M NaCl | pH 7.4 0.0857 M NaCl | pH 7.7 0.0857 M NaCl | pH 8.0 0.0857 M NaCl | pH 6.5 0.0857 M NaCl | pH 6.8 0.0857 M NaCl | pH 7.1 0.0857 M NaCl | pH 7.4 0.0857 M NaCl | pH 7.7 0.0857 M NaCl | pH 8.0 0.0857 M NaCl |
H | pH 6.5 0.1 M NaCl | pH 6.8 0.1 M NaCl | pH 7.1 0.1 M NaCl | pH 7.4 0.1 M NaCl | pH 7.7 0.1 M NaCl | pH 8.0 0.1 M NaCl | pH 6.5 0.1 M NaCl | pH 6.8 0.1 M NaCl | pH 7.1 0.1 M NaCl | pH 7.4 0.1 M NaCl | pH 7.7 0.1 M NaCl | pH 8.0 0.1 M NaCl |
