Fig. 8: Detection of ferroptosis in 1d after UV irradiation.
From: 1,3a,6a-Triazapentalene derivatives as photo-induced cytotoxic small fluorescent dyes
a HeLa cells were cultured with 10, 20 μM and 50 µM of 1d for 1 h with or without 5 µM Ferrostatin-1 (Ferroptosis inhibitor), and then the cells were analyzed by WST8 assay. b HeLa cells were cultured with 10, 20 μM and 50 µM of 1d for 1 h with or without 5 µM Ferrostatin-1 (Ferroptosis inhibitor), and then the cells were analyzed by WST8 assay with UV irradiation. c HeLa cells were cultured with 10 µM of 1d and 5 µM of Mito-FerroGreen for 1 h, and treated with UV irradiation. After irradiation, the cells were perfused and stained Hoechst33342 (Blue), and observed by confocal microscopy. Scale bar = 50 μm. ***P <0.001 (vs Untreated),††P <0.01, †††P <0.01 (vs with Ferrostatin-1) N = 5 biologically independent samples. The error bars represent the standard deviation of the mean.
