Fig. 1: Comparison between standard and crosslinking mRNA display.
From: Identification of photocrosslinking peptide ligands by mRNA display
Previous works describe the use of mRNA display to select reversible ligands to different proteins of interest from mRNA-displayed peptide libraries. In this work, by introducing a photocrosslinking amino acid, p-benzoyl-l-phenylalanine (Bpa) into peptides in the library and denaturing washes during the affinity panning selection step we describe a photocrosslinking methodology linked to mRNA display (XL-RaPID) to find macrocyclic peptides that bind covalently to a protein of interest. Modified steps are highlighted in red.
