Fig. 3: Photocrosslinking of BRDs with pBpa-containing ligands.
From: Identification of photocrosslinking peptide ligands by mRNA display
a Crosslinking between BRD3-BD2 (20 µM) and cyclic peptides (40 µM) after exposure to UV irradiation (365 nm) for variable amounts of time (0, 5, 10, 20, 40 min). Samples were separated on an SDS-PAGE gel and visualised by Coomassie staining. b Plot of percentage of cross-linked BRD3-BD2 calculated by densitometric analysis of protein bands from (a). Crosslinking efficiencies are represented as the mean of triplicate experiments ±1 standard deviation. c Crosslinking of BRD3-BD2/BRD2-BD2/BRD4-BD2/BRD3-BD1 (2 µM) by cyclic peptides (20 µM) after exposure to UV irradiation for 30 min. Samples were analysed and processed as described in (a, b). Crosslinking efficiencies are represented as the mean of triplicate experiments ±1 standard deviation.
