Fig. 4: FAM cyclic peptides selectively label BRD3-BD2 in complex mixtures.
From: Identification of photocrosslinking peptide ligands by mRNA display
a Mixtures containing BRD3-BD2 (10 µM, MW: 16,962) and peptide (2 µM, Bpa-P1-FAM/Bpa-P2-FAM) with/without the presence of BSA (20 µM, MW: ~66,000) were irradiated at 365 nm for 1 h. Samples were separated on an SDS-PAGE gel and visualised by in-gel fluorescence and Coomassie staining. Control experiments were performed without UV irradiation. b Photoaffinity labelling of BRD3-BD2 (1 µM) spiked into cell lysate (45 µg in 10 µL, HEK 293T cells). BRD3-BD2 was preferentially labelled by various concentrations of peptide (4, 2 or 1 µM) after UV exposure (1 h). Control experiments were performed without UV irradiation. Samples were analysed as described in (a).
