Table 2 Calcium sensors discussed in this review.

From: Combining nitric oxide and calcium sensing for the detection of endothelial dysfunction

Number

Name

Kd, µM

Selectivity to Ca2+ against Mg2+, Zn2+ and Na+

λem, nm

pH

Stability in cell environment

Turn-on sensor (ON)/Turn-off (OFF)

Ref.

18

BAPTA sensor family

0.15; ~0.70a

Selective

Fluorophore dependent

Cytosolicd

Stable

ON

192

19

256b

Selective

488

ON

161

20

BCaM

90

Selective

450

Cytosolicd

Stable

ON

193

21

Bichromophore

14.8

Selective

436

ON

194

22

Calix[4]arene

121b

Selective

510

ON

162

23

2400

Selective

550

8.1

ON

163

24

PIAQ–AC 5a

0.309b

Not selective

515

ON

164

25

PIAQ–AC 5b

0.069b

Not selective

515

ON

164

26

0.0192

 

485c

 

165

27

DMK

0.0144

Selective

390

4

ON

195

28

ISB

LOD = 0.3

Selective

483

7.2

ON

166

29

MPFCP-1

0.44

Selective

525

Cytosolicd

Stable

ON

167

30

MPFCP-2

1.21

Selective

525

Cytosolicd

Stable

ON

167

31

BODIPY-BAPTA

0.501b

Selective

508

7.2

Stable

ON

168

32

JF549-BAPTA

0.31

Selective

569

Cytosolicd

Stable

ON

169

33

CaSIR-1

0.58

Selective

>650

Cytosolicd

Stable

ON

170

34

5120

Selective

655

OFF

171

35

Di-Aza-crown-cNDI

0.11b

Selective against Mg2+ and Na+

655

ON

172

36

CaRB

1.22

Selective

631

7.2

7.2

ON

173

37

Calix[4]arene-BDP

Selective against Mg2+

507

OFF

180

  1. FL fluorescence, not mentioned in the original article.
  2. aKd with the presence of competitive ions such as Mg2+.
  3. bRecalculated from reported data.
  4. cλem before adding Ca2+ ions.
  5. dSensor was tested in vitro, pH is in the range 7.2–7.4.