Table 1 Center frequencies from Gaussian fits to C-D spectra and corresponding Em valuesa.

From: The structure of plastocyanin tunes the midpoint potential by restricting axial ligation of the reduced copper ion

 

νas [d2Cys89] (cm−1)

νs [d3Met97] (cm−1)

Em (mV, vs. NHE)

Cu(II)

Cu(I)

Cu(II)

Cu(I)

wt

2235.1 (0.2)

2209.4 (0.3)

2126.30 (0.02)

2123.31 (0.05)

362 (10)

S9A

2234.9 (0.4)

2209.7 (0.1)

2126.30 (0.01)

2123.8 (0.1)

371 (7)

N34A

2235.2 (0.3)

2209.81 (0.08)

2126.50 (0.03)

2123.92 (0.08)

389 (9)

N33A

2234.7 (0.3)

2208.8 (0.4)

2126.23 (0.03)

2124.3 (0.1)

440 (6)

  1. aStandard deviation from triplicate data given in parentheses.
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