Fig. 2: Monosaccharide analysis via HPAEC-PAD of glycovariants of the SARS-CoV-2 spike protein. | Communications Chemistry

Fig. 2: Monosaccharide analysis via HPAEC-PAD of glycovariants of the SARS-CoV-2 spike protein.

From: Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine development

Fig. 2

Results reported in mol monosaccharide mol−1 monomer (protomer MW = 143,230.6 Da). Results are means of three triplicate injections and three triplicate reactions. Error bars are the standard deviation observed between the triplicate hydrolyses values. a Fuc, GlcN, Gal, and Man content obtained after TFA hydrolysis. The data were analyzed using two-way ANOVA, Tukey’s multiple comparisons test, comparing the content of each monosaccharide type between variants. Not all comparisons shown. For full comparisons see Supplementary Table 2. b Neu5Ac content obtained after enzymatic hydrolysis. For a full comparison see Supplementary Table 3. P values: <0.03 (*), <0.002 (**), and <0.0001(****).

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