Fig. 6: PET46 is a thermostable hydrolase adapted to the conditions of the Guaymas Basin.
From: An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate
The enzyme’s optimal temperature and pH were determined by incubation with pNP-ester substrates (decanoate, C10, a). The enzyme conserved most of its activity after 8-day incubation at 60 °C, but lost almost 80% after two days at 70 °C b. NanoDSF experiments show that PET46 has a melting temperature (Tm) of 84.55 °C c. Error bars indicate the standard deviation (n = 3). Standard deviation in “a” was below 6% for all conditions assayed. Supporting data can be found in Supplementary Data 2.
