Table 3 PET46 degrades semi-crystalline PET powder at high temperatures.

From: An archaeal lid-containing feruloyl esterase degrades polyethylene terephthalate

Enzyme

T [°C]

TPA [µM]

MHET [µM]

BHET [µM]

Σ [µM]

Conv. [%]

PET46

 

1.91 ± 0.77

0 ± 0

0 ± 0

1.91 ± 0.77

0.00

IsPETase (*)

30

1250.56 ± 166.54

49.41 ± 11.23

0 ± 0

1,299.97 ± 166.91

2.71

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3.75 ± 9.44

0.35 ± 0.3

0.13 ± 0.04

4.23 ± 9.45

0.01

PET46

 

790.41 ± 206.22

0.85 ± 0.82

0.14 ± 0.06

791.4 ± 206.22

1.65

LCC (*)

50

1627.57 ± 322.23

2147.9 ± 286.1

57.21 ± 8.3

3,832.67 ± 430.99

7.98

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6.27 ± 7.89

0.68 ± 0.14

0.14 ± 0.03

7.09 ± 7.89

0.01

PET46 (*)

 

1609.25 ± 260.17

14.44 ± 2.07

0.45 ± 0.13

1,624.14 ± 260.18

3.38

LCC

60

1922.12 ± 47.76

505.29 ± 110.34

13.26 ± 3.46

2,440.68 ± 120.28

5.08

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7.37 ± 1.02

0.86 ± 0.18

0.2 ± 0.09

8.44 ± 1.04

0.02

  1. Product release measurements underlying Fig. 5a show that the WT enzymes PET46, IsPETase, and LCC release comparable amounts of aromatic compounds after three days at their optimal temperatures (indicated with an asterisk). Percentage of conversion assumes an initial concentration of 48 mM TPA equivalents.