Fig. 7: Gradual dehydration of cells in the isotonic polyZI solution. | Communications Chemistry

Fig. 7: Gradual dehydration of cells in the isotonic polyZI solution.

From: Cell-compatible isotonic freezing media enabled by thermo-responsive osmolyte-adsorption/exclusion polymer matrices

Fig. 7: Gradual dehydration of cells in the isotonic polyZI solution.

Relative BOSC cell volume in 10% (w/v) polyZI/1.0% (w/v) NaCl aq. and 1.0% (w/v) NaCl aq. at the indicated temperatures (standardized as 100% at room temperature) when cooling at −1 °C/min (n = 3, biological triplicates, one-way ANOVA). These cells were incubated as floating cells after trypsinization. The final cell volume was measured immediately before freezing as measurement becomes difficult after freezing.

Back to article page