Fig. 9: Remove polyZI after cryopreservation.

Number of living BOSC cells and mNF immediately and 5–7 days after thawing and washing with the indicated solutions (n = 3, biological triplicates, one-way ANOVA). Commercial: Culture Sure freezing medium from Fujifilm Wako Pure Chemical Corporation. Trypsin solution: cells were added to trypsin solution (0.5 w/v% trypsin-5.3 mmol/L EDTA・4Na solution without phenol red (×10), Fujifilm Wako Pure Chemical Corporation)) that had been diluted 10 times with phosphate buffered saline (PBS) and incubated for 3 min before centrifugation.