Fig. 8: C3N-Dbn-Trp2 partially inhibits ABCG2, but not ABCC1.
From: Exploring bat-inspired cyclic tryptophan diketopiperazines as ABCB1 Inhibitors
a–c Western blot analysis of ABC family proteins in the indicated parental cells and ABCC1 knockout cells. Actin was used as a loading control. d, e Flow cytometry analysis of CMFDA accumulation. HEK293T parental and ABCC1 knockout cells d, and HCT-15 ABCB1 knockout cells and ABCB1/ABCC1 double knockout cells e were pre-treated with 10 µM C3N-Dbn-Trp2 before incubating with 1 µM CMFDA. Bar graphs represent the mean ( ± SD of three independent experiments) fluorescent intensity of CMFDA relative to the DMSO-treated parental cells (the first bar). f Western blot analysis of ABCG2 in BeWo parental cells and ABCG2 knockout cells. Actin was used as a loading control. g Flow cytometry analysis of Hoechst 33342 accumulation. BeWo parental and ABCG2 knockout cells were pre-treated with 10 µM Ko 143 or C3N-Dbn-Trp2 before incubating with 3 µM Hoechst 33342. Bar graphs represent the mean ( ± SD of three independent experiments) fluorescent intensity of Hoechst 33342 relative to the DMSO-treated parental cells (the first bar).
