Fig. 2: Directed recruitment of the c-Raf phosphopeptide into coacervates with the highest affinity isoform of 14-3-3.

a Schematic overview of binding of the FITC-c-Raf pS peptide to 14-3-3 isoforms and uptake of FITC-c-Raf pS in individual populations of coacervates containing either the weak 14-3-3σ isoform or the strong 14-3-3γ isoform. b Confocal micrographs showing the uptake of FITC-c-Raf pS in individual populations in the absence or presence of different 14-3-3 isoforms. Scale bar: 25 µm. Uncropped images are available in Supplementary Fig 3. c Schematic overview of competitive uptake of FITC-c-Raf pS into the coacervates containing two 14-3-3 isoforms with different affinity. d Confocal micrograph of competitive FITC-c-Raf pS uptake in a mixed coacervate sample containing coacervates loaded with either 14-3-3σ or with 14-3-3γ. Scale bar: 25 µm. Uncropped images are available in Supplementary Fig. 3. e, f Quantification of the FITC-c-Raf pS signal from micrographs of individual populations (e) or mixed (f) coacervate populations containing different 14-3-3 isoforms. Statistical differences were analyzed by nonparametric Dunn’s test with correction for multiple comparisons, with N ≥ 21 coacervates across multiple imaging positions in the same sample. P values are shown above the comparison. Dashed lines represent the median and dotted lines represent the upper and lower quartiles. ns: no statistical difference. The fold difference is given as the fold difference between the means.