Fig. 5: Sequence minimization testing for Clone 1 and Clone 2.

Deduced minimized structures are shaded within the full-length construct. E denotes EdU. T in the minimized structures represents EdU bases, where T was used as a base surrogate for mFold structure prediction. A qualitative score from + (weak covalent conjugation) to +++++ (strong covalent conjugation) was assigned to each construct based on binding assays detailed in Supplementary Figs. S3 and S4.