Fig. 3: Structural and functional comparisons of p97 with various triazole inhibitors. | Communications Chemistry

Fig. 3: Structural and functional comparisons of p97 with various triazole inhibitors.

From: Mechanism of allosteric inhibition of human p97/VCP ATPase and its disease mutant by triazole inhibitors

Fig. 3

a NMS-873 binding site (PDB code: 7LMY). Orange-red is the p97 D2 domain. b UPCDC30245 binding site (PDB code: 5FTJ). c Structural comparison of the nucleotide-binding sites between p97|ADP,NSC799462 and p97E578Q | ATP,NMS-873. Yellow, blue, and pink sticks are the intersubunit signaling (ISS) motif, arginine fingers, and E578 (p97|ADP,NSC799462) or Q578 (p97E578Q|ATP,NMS-873). Distances are measured in Å. d Histogram comparing IC50 measurements (on a logarithmic scale) of triazole inhibitors and CB-5083 on the ATPase activities of various purified p97 mutants (n = 6). Data are presented as the mean ± standard deviation (SD). CB-5083 is an ATP-competitive inhibitor. WT represents the wild type.

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