Fig. 2: Portia tree extract is the most promising Kv7.2/3 opener among the 7 extract hits.

a Images of plants and alga from which the top 7 hits were extracted. b Exemplar Kv7.2/3 traces recorded from oocytes in the absence (Control) or presence of the extracts indicated at 1:50 dilution (5 mg fresh plant matter starting material/ml) using the voltage protocol indicated (inset). Scale bars lower left for each pair of traces; n = 5 (stickseeds, saltbush, Spanish needle), 7 (portia tree), 9 (pussypaws), 11 (sugar bush hybrid), 12 (kelp) biologically independent oocytes per group. c Mean peak prepulse current for traces as in (b); n values as in (b), in the absence (black) or presence (red) of plant extracts shown on left. d Mean peak tail current for traces as in (b) (measured at arrows shown in b); n values as in (b), in the absence (black) or presence (red) of plant extracts shown on left. e Mean normalized tail current (G/Gmax) for traces as in (b) (measured at arrows shown in b); n values as in (b), in the absence (black) or presence (red) of plant extracts shown on left. f Mean unclamped oocyte membrane potential for Kv7.2/3-expressing oocytes as in (b); n values as in (b). Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by t-test. Dashed lines indicate zero current level. Blue boxes contain tail currents for the corresponding traces expanded to preserve scale (test vs control).