Fig. 3: Screening of portia tree metabolites reveals gentisic acid as the principal Kv7.2/3-activating compound. | Communications Chemistry

Fig. 3: Screening of portia tree metabolites reveals gentisic acid as the principal Kv7.2/3-activating compound.

From: Discovery of a potent, Kv7.3-selective potassium channel opener from a Polynesian traditional botanical anticonvulsant

Fig. 3

a Mean peak tail current (left) and normalized tail current (G/Gmax) (right) for Kv7.2/3 channels expressed in oocytes, in the absence (black) or presence (red) of portia tree metabolites indicated (100 µM); n = 6 (coumarin, malic acid), 7 (catechin hydrate, mucic acid), 8 (citric acid), 10 (gentisic acid) per group. b Mean unclamped oocyte membrane potential for Kv7.2/3-expressing oocytes as in (a); n values as in panel (a) except coumarin (n = 5). CH catechin hydrate. c Mean peak tail current (left) and normalized tail current (G/Gmax) (right) for Kv7.2/3 channels expressed in oocytes, in the absence (black) or presence (red) of portia tree metabolites indicated (100 µM); n = 5 (naringenin, proline), 7 (protocatechuic acid, rac-Glycerol 1-myristate), 8 (sinopic acid), 9 (vanillic acid) per group. d Mean unclamped oocyte membrane potential for Kv7.2/3-expressing oocytes as in (c); n values as in panel (c). PA protocatechuic acid. Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by t-test. Black dashed lines indicate zero current level.

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