Fig. 5: Gentisic acid is highly selective for Kv7.3 over the other Kv channels tested.

a Exemplar traces recorded from oocytes expressing the Kv channels indicated in the absence (Control; black) or presence of gentisic acid (100 µM) (red) using the voltage protocol indicated (inset). Scale bars lower left for each pair of traces; n = 6 (Kv7.2/5, Kv7.3/5), 7 (Kv7.1, Kv7.2/3/5), −9 (Kv7.4). b Mean peak prepulse current (left) and normalized tail current (G/Gmax) (right) for traces as in (a); n values as in panel (a), in the absence (black) or presence (red) of gentisic acid (100 µM). c Mean unclamped oocyte membrane potential for oocytes as in (a); n = 6–9 per group. d Exemplar traces recorded from oocytes expressing Kv7.1/KCNE3 in the absence (Control; black) or presence of gentisic acid (100 µM) (red) using the voltage protocol indicated (inset). Scale bars lower left for the pair of traces; n = 10 per group. e Mean peak prepulse current (left) and normalized tail current (G/Gmax) (right) for traces as in (d); n = 10 per group, in the absence (black) or presence (red) of gentisic acid (100 µM). f Mean unclamped oocyte membrane potential for oocytes as in (d); n = 10 per group. g Exemplar traces recorded from oocytes expressing Kv1.1, Kv1.2 or Kv2.1 as indicated in the absence (Control; black) or presence of gentisic acid (100 µM) (red) using the voltage protocol indicated (inset). Scale bars lower left for the pair of traces; n = 8 per group. h Mean peak prepulse current (left) and normalized tail current (G/Gmax) (right) for traces as in (g); n = 8 per group, in the absence (black) or presence (red) of gentisic acid (100 µM). i Mean unclamped oocyte membrane potential for oocytes as in (g); n = 8 per group. Error bars indicate SEM. n indicates number of biologically independent oocytes. Statistical comparisons by t-test. Dashed lines indicate zero current level.