Fig. 1: Activation of FlashCap-eGFP-mRNA translation at 6 h and 12 h post-transfection.
From: Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs
A Photolysis reaction of the FlashCap-mRNA. B Schematic illustration of light-mediated activation of translation. Irradiation was performed at different timepoints. C Fluorescence confocal microscopy images of HeLa cells transfected with FlashCap-eGFP-mRNA. Cells were either kept in the dark or irradiated (365 nm, 15 s) at indicated timepoints (6 h or 12 h) after transfection. Cap0-eGFP-mRNA serves as a positive control and was kept in the dark. Samples were imaged for eGFP fluorescence 24 h after transfection. Shown is one representative set of n = 3 independent replicates.
