Fig. 4: Activation of mRNA translation with spatiotemporal control in developing zebrafish embryos.
From: Spatiotemporal control of translation in live zebrafish embryos via photoprotected mRNAs
A Schematic illustration of light-mediated local activation of translation in zebrafish embryos. FlashCap-mRNA is injected at the one-cell stage. Local irradiation in the trunk area is initiated at 24 hpf using the confocal laser scanning microscope and a 405 nm laser. The zebrafish embryo is then imaged at 48 hpf. B Z-projection of the GFP channel in the trunk region of an embryo at 48 hpf. Left: After local irradiation of the indicated area in the trunk (boxed area) of a FlashCap-eGFP-mRNA injected zebrafish embryo at 24 hpf, a strong induction of eGFP expression is evident at 48 hpf. Right: False-colored version of the GFP channel depicting different pixel intensities in different colors and highlighting the strong induction of eGFP expression in the irradiated area. Shown is one representative experiment of n = 3 independent replicates.
