Fig. 2: The working hypothesis of this research.

The established structure of a psoralen-conjugated triplex-forming oligonucleotide (TFO) with the proposed design of (1’(one)-psoralen-conjugated triplex-forming oligonucleotide (OPTO)) is shown. The optimized orientation of psoralen in OPTO is better for intercalating psoralen into the target DNA.