Fig. 5: The UV-melting profiles of the triplex of L-OPTO with varied sequences. | Communications Chemistry

Fig. 5: The UV-melting profiles of the triplex of L-OPTO with varied sequences.

From: Development and functional evaluation of a psoralen-conjugated nucleoside mimic for triplex-forming oligonucleotides

Fig. 5

a Sequences of four PPT duplexes (Py-1/Pu-1, Py-2/Pu-2, Py-3/Pu-3, and Py-4/Pu-4), LNA-incorporated TFO (L-TFO2–5), and LNA-incorporated OPTO (L-OPTO5–8). b Normalized UV-melting curves of each triplex at pH 7.0 and their respective Tm values. The Tm values of L6-8 were determined by differential method from the region between the dotted line. The final concentration of duplex and TFO was 3 μM each. The Tm values are displayed as the mean ± s.d. for n = 3 replicates. c Native PAGE of the triplexes containing L-OPTO6–8 (L6–8) at pH 7.0 and 37 °C. The final concentration of duplex and TFO was 1 μM each. The bands were visualized using SYBR® Gold stain.

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