Fig. 6: The photo-cross-linking properties of L-OPTO. | Communications Chemistry

Fig. 6: The photo-cross-linking properties of L-OPTO.

From: Development and functional evaluation of a psoralen-conjugated nucleoside mimic for triplex-forming oligonucleotides

Fig. 6

a Photo-cross-linking of psoralen with the pyrimidine base and the sequence of the PPT duplexes. The correlation between the cross-linking efficiency and the number of psoralen molecules that were introduced into the TFO sequence (L-OPTO2-4) was examined. b The photo-irradiation (365 nm) was conducted at pH 7.0 and 37 °C. The final concentration of duplex and TFO was 1 μM each. The samples were collected at each irradiation time (0, 1, 5, 10, 15, 20, 25, and 30 s), and the product formation was analyzed by denaturing P. The 3’ end of the L-OPTOs was labeled with tetramethylrhodamine (TAMRA). The graphs show the quantification results of the photo-cross-linking efficiency of each sequence. The data are displayed as the mean ± s.d. for n = 3 replicates.

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