Fig. 6: Proposed reaction mechanism of modified nucleosides with hydrazine supported by high-resolution mass spectra and MS2/MS3 fragmentation spectra.
From: Chemical reactivity of RNA and its modifications with hydrazine
Reaction mechanisms are given above the MS1 spectrum of the intact nucleoside product (left), MS2 spectra of the nucleobase product (middle) and MS3 spectra of the fragmented nucleobases (right). The fragmentation reactions of the nucleobases are indicated in red within the MS3 spectra and in the nucleobase structure given above (blue boxes). a 4-thiouridine (s4U) b dihydrouridine (D) c 7-methylguanosine (m7G).
