Fig. 1: Identification of Gal peptide isomers in rat hypothalamic extract by LC-TIMS-MS. | Communications Chemistry

Fig. 1: Identification of Gal peptide isomers in rat hypothalamic extract by LC-TIMS-MS.

From: Isoaspartate-containing galanin in rat hypothalamus

Fig. 1

a Extracted ion chromatogram (EIC) of endogenous Gal (m/z 791.65; z = 4+). Extracted ion mobilogram (EIM) of Gal peak 1 (rt = 44.5 min) black trace and peak 2 (rt = 46.5 min) red trace at (b) z = 3+, (c) z = 4+, (e) z = 5+, (f) z = 6+ A. (d). EIC of isotopically labeled Gal spiked into hypothalamic tissue homogenate (m/z 793.9; z = 4+); black trace, EIC of endogenous Gal (m/z 791.65; z = 4+); red trace. Each plot inserts is the corresponding MS spectra at the specified charged state. (g). Top panel; EIC of endogenous Gal from hypothalamic extract (m/z, 791.65; z = 4+), middle panel; Black trace: EIC of isotopically labeled Gal synthetic standard (m/z = 793.90; z = 4+), Red trace: EIC of D17IsoAsp-Gal standard (m/z = 791.65; z = 4+), bottom panel; black trace: EIC of endogenous Gal (m/z = 791.65; z = +4), red trace: EIC of Gal after spiking D17IsoAsp-Gal standard into extracts. (h). Top panel; Black trace EIM of Gal peak1 in (a) top panel (m/z = 633.52; z = 5+, rt = 27.5 mins), red trace; EIM of Gal peak2 in (a) top panel (m/z = 633.52; z = 4+, rt = 28.3 mins), bottom panel; black trace: EIM of isotopically labeled Gal synthetic standard (m/z = 635.32; z = +5), red trace: EIM of D17IsoAsp-Gal standard (m/z = 633.52; z = +5).

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