Fig. 3: In vitro isomerization of Gal.

a EIC of Gal at different incubation times. b A plot of the percentage of IsoAsp-containing Gal signal to total Gal signal (rt = 29.0 mins) against incubation time. Isomerization was performed in phosphate buffer pH 5.8 and at 37 °C. Percent IsoAsp was calculated as the area of (peak2/(peak1+peak2)) *100. Error bars in (b) represent the standard deviation of n=3 replicates.