Fig. 4: Characterization of Gal and D17IsoAsp-Gal fibrillization.

a. Thio T assay-determined Gal and D17IsoAsp-Gal fibrillization in presence or absence of heparin. b Effect of PIMT on Gal amyloid fibril formation in the presence of heparin. c LC-MS analysis of Gal and heparin (black trace) and D17IsoAsp-Gal and heparin (red trace) mixtures after 2 weeks of incubation. d Thio T fluorescence kinetics of Gal and D17IsoAsp-Gal amyloid fibril formation and the impact of PIMT on the kinetics of D17IsoAsp-Gal fibrillation. Each data point in (d) corresponds to the average from three measurements normalized using maximum normalization. e TEM images of Gal (top panel) and D17IsoAsp-Gal (lower panel) after fibrillization reaction with or without heparin and PIMT. The statistical analysis in (a) and (b) was performed using Welch’s ANOVA (F = 282.4, P = 0.00035) followed by Games-Howell pairwise comparison and two-sample t-test with Welch’s correction respectfully. Error bars represent standard deviations of n=3 replicates.