Fig. 1: Synthesis and uncaging of MNI-l-lac.
From: Synthesis and application of a photocaged-l-lactate for studying the biological roles of l-lactate
a Synthetic scheme of MNI-l-lac. b Schematic representation of the photo-uncaging of MNI-l-lac to release l-lactate, a proton, and the nitrosoindole by-product. c Absorbance spectra were recorded during the progressive photolysis of MNI-l-lac (40 μM solution in l-lactate (-) buffer (30 mM MOPS, 100 mM KCl, 1 mM CaCl2, pH 7.2) containing 10% DMSO). Dotted red curve: MNI-l-lac, λmax (ε) = 330 nm (5400 M−1cm−1). Black curve: nitrosoindole by-product after photolysis, λmax (ε) = 403 nm (7200 M−1cm−1). The arrows indicate the effect of increasing irradiation time for photolysis.
