Fig. 2: In vitro characterization of MNI-l-lac.
From: Synthesis and application of a photocaged-l-lactate for studying the biological roles of l-lactate
a Schematic representation of the eLACCO1 biosensor’s fluorescence response. eLACCO1 consists of cpGFP + l-lactate binding domain (LBD). b eLACCO1 fluorescence emission (λex = 460 nm) with MNI-l-lac (40 µM) with different UV illumination times (average of n = 3). The arrow indicates the effect of increasing illumination time. c Overlay of l-lactate (gray) and uncaged MNI-l-lac (black) titrations using the eLACCO1 fluorescence biosensor (n = 3, error bars are SEM). MNI-l-lac solutions in varying concentrations were illuminated for 10 min.
