Fig. 4: GPR81 activation by MNI-l-lac photoactivation.
From: Synthesis and application of a photocaged-l-lactate for studying the biological roles of l-lactate
a Schematic showing the activation of GPR81-cAMP signaling pathway from uncaging MNI-l-lac. b Images of the cAMPinG1 expressing HeLa cells before and after photoactivation at 1 min (405 nm, 1 s, 2.4 mW/cm2). Photoactivation and pseudocoloring (ΔF/F column) were done as described in Fig. 3b with F0 at t = 0.8 min and F at t = 5 min. Scale bars represent 20 µm. c Fluorescence response of cAMPinG1 versus time in HeLa cells expressing both cAMPinG1 and GPR81-mCherry in the presence (solid, cyan line) or absence (dashed, grey line) of 5 mM MNI-l-lac. n = 24 for both conditions (n = 3 biological replicates, mean ± SEM).
