Fig. 1: Semi-synthesis of TDP-43.

a Previously reported C-terminal LCD-modified TDP-43 fragments. me: N-methylated peptide bond; pS: phosphorylated Ser. b Full-length TDP-43 possessing pS48 (designated as TDP1-414[pS48]) synthesized in this work. c Synthetic scheme of TDP1-414(pS48). MeNbz (N-acyl-N′-methyl-benzimidazolinone) is an N-acylurea linker that functions as a thioester surrogate21. CBD: chitin-binding domain.