Fig. 5: DNP-ssNMR spectra of the Aβ1-42-membrane samples at intermediate incubation times in POPC liposomes. | Communications Chemistry

Fig. 5: DNP-ssNMR spectra of the Aβ1-42-membrane samples at intermediate incubation times in POPC liposomes.

From: Structural convergence and membrane interactions of Aβ1-42 along the primary nucleation process studied by solid state NMR

Fig. 5

All ssNMR samples contain selective-13C isotope labeling at residues E3, V12, F19, A21, I32, L34 and G38. A 2D 13C-13C DARR spectra with 250 ms mixing time. Colored rectangles highlight the spectral regions from which the 1D slides are extracted. B 1D slides that represent the 13C chemical shifts in the t1 dimension of the rectangle regions. Color-coding for panels (A, B): red, intra-residue cross peaks; blue, inter-residue cross peaks; purple, cross peaks between 13C-sites in Aβ1-42 and natural abundance 13Cs in lipids. C 2D DQ-SQ correlation spectra acquired on the same samples as for the DARR spectra. Colored-lines in the 15-h spectrum highlight the intra-residue aliphatic cross peaks for individual labeled residues. All spectra were processed with 50 Hz Gaussian line broadening in each dimension.

Back to article page