Fig. 2: Topological polar surface area plot of commonly used crosslinker reagents.
From: Developing a new cleavable crosslinker reagent for in-cell crosslinking
DiSPASO is shown in the center of the plot (green dot) with moderate membrane permeability and hydrophobicity compared to other common crosslinker regents. DiPPASO (not presented in this study) shows similar properties to t-BuPhoX (TBDSPP) with a high hydrophobicity compared to other crosslinkers. The clouds of enrichable and permeable crosslinkers are clustered in two distinct parts of the plot, with DiSPASO at the interface between both groups. Classification of the crosslinkers was extracted from the Thermo Scientific crosslinker selection tool. If a crosslinker shows two properties of the classifications the more dominant property was selected for plotting.
