Fig. 8: Quantitation of crosslink fluorescence signals within nuclei of DiSPASO vs. DSBSO microscopy images.
From: Developing a new cleavable crosslinker reagent for in-cell crosslinking
A Quantitation of the green Alexa 488 signal of DiSPASO crosslinked proteins. The fluorescence intensity increases fast within the first 5 min after reaching a plateau at 30 min. Each dot represents a signal intensity of crosslinked proteins in a nucleus. B Nuclei control signal intensity of DAPI. The background intensity of DAPI is low in comparison to the intensity of crosslinked proteins. C Quantitation of the red Alexa 555 signal of DSBSO crosslinked proteins. The fluorescence intensity increases slowly and reaches its maximum at 30 min. D Nuclei control signal intensity of DAPI. The background intensity of DAPI is also low in comparison to the intensity of crosslinked proteins. Quantitation of signal intensities of all images was performed in Fiji ImageJ (version 1.54f). The number of quantified nuclei is indicated as separate black dots on top of the bar. The standard deviation was estimated as average distance from each data point to the sample mean.
