Fig. 1: Deposition of ageing FUS condensates via microfluidic spraying and characterisation of condensate material properties.
From: Nanoscale profiling of evolving intermolecular interactions in ageing FUS condensates
Overview of the experiments employed in this study. Condensates were deposited using a microfluidic spray device, which enables their characterisation via surface-based techniques. Four properties of condensates were probed as a function of ageing: (1) 3D morphology via AFM, (2) hydrogen-deuterium exchange rates via IR spectroscopy, (3) material properties via AFM nanomechanical mapping, and (4) chemical and structural properties via IR spectroscopy. This combined approach allowed us to generate a detailed model of how phases emerge in space and time within FUS condensates, and how these phases correlate with conformational changes at the polypeptide level.
