Fig. 7: In vivo targeting of intraArg-TRM to mouse ears.
A In vivo fluorescence images of the mice treated with intraArg-TRM. The DiD-loaded micelles were injected into mouse tail veins (5.0 mg/mL, 200 µL) and then the left ear of each mouse was heated at 42 °C for 30 min. The colored scale bar indicates the intensity of fluorescence of DiD in radiant efficiency expressed as (photons/s/cm2/steradian)/(µW/cm2). B Accumulation of the arginine-introduced thermo-responsive micelles in the ears. At 24 h after the injection, mouse ears were collected and homogenized. The accumulation rate of micelles was calculated from the fluorescence intensity of DiD in tissue lysates, and the horizontal lines represented the median (n = 7, Mann–Whitney U test). C Confocal microscopic images of the left ear of the mice treated with intraArg-TRM. The ear was collected at 24 h after the injection and frozen sections were prepared for the observation. DIC: differential interference contrast, Red: DiD-loaded micelles, blue: DAPI, green: CD31 (blood vessels); scale bars: 50 μm.
