Fig. 5: The microscopic steps of α-syn amyloid aggregation.
From: Surface wetting is a key determinant of α-synuclein condensate maturation
a Dispersed solution aggregation of FL (blue), 5-140 (red), 11-140 (green) and 19-140 (purple) α-syn. Three individual biological repeats are shown. Each repeat is the mean of either two or three normalized technical replicates. The error bars represent the standard deviation of the mean. Bar plots representing the mean Hillslope (b) and tlag (c) values of FL (blue), 5-140 (red), 11-140 (green), and 19-140 (purple) α-syn. Error bars represent the standard error of the mean of n = 3 biological repeats, with individual data points represented by crosses. Statistical significance was determined using a Welch and Brown-Forsythe ANOVA with multiple comparisons against FL α-syn. p-values for 5-140, 11-140, and 19-140 α-syn were <0.0001, 0.0273, and 0.0002 for b and 0.0036, 0.0267, and 0.9235 for c, respectively. d Representative TEM images of the insoluble fraction after aggregation. Short fibrils are highlighted by a red asterisk and fibril twists are indicated by an arrow. Scale bars represent 500 nm. e Aggregation kinetics of FL (blue), 5-140 (red), 11-140 (green) and 19-140 (purple) α-syn under conditions promoting lipid-induced primary nucleation (top), elongation (middle), or secondary nucleation (bottom). A single representative biological repeat is shown. Each repeat is the mean of three technical replicates. The semi-transparent error bars represent the standard deviation of the mean.
