Fig. 1: Metabolic engineering of S. eitanensis enhanced (−)-premarineosin A (3) production. | Communications Chemistry

Fig. 1: Metabolic engineering of S. eitanensis enhanced (−)-premarineosin A (3) production.

From: Metabolic engineering and late-stage functionalization expand the chemical space of the antimalarial premarineosin A

Fig. 1: Metabolic engineering of S. eitanensis enhanced (−)-premarineosin A (3) production.

a Comparison of the S. eitanensis premarineosin A BGC and the Streptomyces sp. CNQ-617 marineosin A BGC using clinker64. The premarineosin A BGC lacks genes with homology to marA and marE (highlighted by the red box). b The final biosynthetic step of the premarineosin A pathway. c Crystal structure of (−)-premarineosin A (3). d (−)-Premarineosin A (3, yellow) and 23-HUP (2, red) production (mg L−1), quantified by AUC (HPLC) against standard curves of each compound. Dots represent three independent culture replicates. Error bars indicate standard deviation (SD). ND: Not Determined, the measured production was below the limit of quantification. e Engineered FP10016 (green) and FP10018 (red) strains cultivated in solid and liquid GICYE media. f (−)-Premarineosin A (3) production (mg) per gram of dry cell weight (DCW, g) over time by wild-type (dark green) and engineered FP10016 (light green), FP10017 (light purple), and FP10018 (dark purple) strains. Symbols represent three independent culture replicates. Error bars indicate standard deviation (SD) for n = 3 biological replicates. g Isolated titers of pure (−)-premarineosin A (3) from the engineered FP10017 (light purple) and FP10018 (dark purple) strains. Dots represent three independent culture replicates. Error bars indicate standard deviation (SD).

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