Scheme 4 | Communications Chemistry

Scheme 4

From: Creating unimolecular multivalent diversity in protein conjugates via the Passerini multicomponent bioconjugation with isocyanoproteins

Scheme 4The alternative text for this image may have been generated using AI.

Passerini bioconjugation for the assembly of multivalent glycoconjugates incorporating bacterial polysaccharide antigens. A Repeating unit of the pneumococcal serotype 14 (Pn14) and the meningococcal serogroup C (MenC) capsular polysaccharides (CPs). B SE-HPLC traces (TSK 5000 PW column) of the natural, fragmented and—for CPs Pn14—oxidized polysaccharides. C Schematic representation of the structures of the multivalent BSA-MenC-Pn14 glycoconjugates produced by the Passerini bioconjugation with isocyanoproteins. D Antigenicity evaluation by Dot blot assays of glycoconjugates 23, 24, 25 and 26 in comparison with isocyano-BSAs and modified CPs, using reference antibodies against BSA, S. pneumoniae and N. meningitidis. Native BSA and the natural CPs antigens were used as positive controls.

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