Table 1 Three-colour FRET model Lifetimes and FRET efficiencies

From: Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts

Purified Protein

Average lifetime (ns)

SEM

Average χ2/d.f.

  

mTurq2

4.178

0.01177

0.949

  

mVenus

3.020

0.03676

1.095

  

mTurq2-mVenus

2.668

0.04371

0.908

  

mVenus-mScarlet

2.393

0.02838

0.991

  

mTurq2-mScarlet

2.600

0.01738

1.089

  

mTurq2-mVenusG68A-mScarlet

3.242

0.03438

1.159

  

mTurq2-mVenus-mScarlet

2.332

0.05397

1.058

  

mTurq2-mVenus-mScarlet

2.509

0.06745

1.075

  

FRET Pair

FRET (%)

SEM

R0 (nm)

R (nm)

SEM

mTurq2-mVenus

35.89

0.564

5.83

6.41

5.23E-02

mTurq2-mVenus-mScarlet

43.72

0.892

n.a.

n.a.

n.a.

mTurq2-mVenusG68A-mScarlet

22.41

0.4572

5.08

6.25

5.49E-02

mTurq2-mScarlet

37.77

0.2739

5.08

5.52

2.15E-02

mVenus-mScarlet

20.78

0.7336

5.53

6.91

1.03E-01

mTurq2-mVenus-mScarlet

16.94

0.6397

5.53

7.21

1.09E-01

  1. Summary tables detailing the fluorescence lifetimes and χ2/degrees of freedom (goodness of fit metric) for the purified proteins used in the FRET cascade model. FRET efficiencies, Forster radii (R0), separation distance (R) and their respective SEM are also presented. N = 9 measurements, cells per condition across three separate technical repeats.
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