Table 2 Three-colour FRET model energy transfers

From: Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts

 

Energy Transfer (s-1)

SEM

Lifetime (ns)

KmTuq2

2.39E + 08

2.81E-08

4.178

KmTuq2-mVenus

3.75E + 08

1.64E-07

2.668

ΓmTuq2-mVenus

1.35E + 08

5.91E-08

7.382

KmTuq2-mVenusG68A-mScarlet-I

3.08E + 08

1.06E-07

3.242

ΓmTuq2-mScarlet-I

6.91E + 07

1.40E-07

14.471

K mTuq2-mVenus-mScarlet-I Calculated

4.44E + 08

2.32E + 07

2.253

KmTuq2-mVenus-mScarlet-I Measured

4.29E + 08

1.47E + 07

2.331

Difference (%)

  

3.36%

  1. A summary table detailing the energy transfer rates, SEM, and the associated lifetime of each energy transfer in the construct. K = energy transfer rate for a specific fluorophore; Γ = specific FRET energy transfer within a FRET pair. N = 9 measurements cells per condition across three separate technical repeats.
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