Table 3 Comparison of Bimodal fitting parameters for VincTS and VincTS + mScarlet-RIAM using the Cascade FRET energy transfer model

From: Development of three-colour FRET cascade for force sensing of the putative RIAM-vinculin interaction in fibroblasts

Lifetime and force bimodal fit parameters for VincTS only

Lifetime and force bimodal fit parameters for VincTS + mScar-RIAM

Lifetime Bimodal Fit Parameters:

Lifetime Bimodal Fit Parameters:

 

Lifetime (ns)

Std. dev.

 

Lifetime (ns)

Std. dev.

x1

1.893

0.039

x1

2.154

0.182

σ1

0.249

0.026

σ1

0.437

0.121

A1

0.838

0.080

A1

0.584

0.993

x2

2.547

0.072

x2

3.146

0.270

σ2

0.305

0.051

σ2

0.406

0.181

A2

0.571

0.048

A2

0.362

0.118

Forces Bimodal Fit Parameters:

Forces Bimodal Fit Parameters:

 

Force (pN)

Std. dev.

 

Force (pN)

Std. dev.

x1

2.737

0.080

x1

3.067

0.0583

σ1

0.413

0.046

σ1

0.409

0.0394

A1

0.678

0.065

A1

0.784

0.0666

x2

3.172

0.019

x2

3.454

0.0219

σ2

0.161

0.030

σ2

0.114

0.0292

A2

0.686

0.140

A2

0.633

0.1345

  1. A summary table detailing the lifetime. Standard deviation and fractional intensity for each peak in the bimodal modal for lifetime and force for VincTS and for VincTS + mScarlet-RIAM. Where \(x\) = the mean; \(\sigma\) = standard deviation, and A = amplitude of the bimodal Gaussians.
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